Device

Part:BBa_J24676

Designed by: Annie Gao   Group: iGEM06_Brown   (2006-10-26)


pBAD to tetR coding region, lacI coding region, and mCherry reporter

Links the pBAD promoter, which is induced by arabinose and inhibited by araC, to a tetR coding region with an RBS, a LacI coding region with an RBS, and an mCherry reporter. Activation of this pathway results in tetR production, lacI production, and mCherry production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//function/regulation/transcriptional
Parameters
None